SBT leaf extract-treated plasma, (for 60 min, p < 0.05 for tested concentrations: 10 and 50 μg/mL). The effects were statistically significant: SBT twig extract-treated plasma vs. The effect of three different concentrations of SBT twig extract (5, 10 and 50 μg/mL for 15 min) was statistically significant (* p 0.05 (n.s.), for all tested concentrations- 0.5 - 50 μg/mL) for 60 min ( p > 0.05 (n.s.), for tested concentrations: 0.5, 1 and 10 μg/mL). The effect of two different concentrations of SBT leaf extract (10 and 50 μg/mL for 15 min) was not statistically significant ( p > 0.05 (n.s.)) in comparison to control. The effect of two different concentrations of two tested extracts (0.5 and 1 μg/mL for 15 and 60 min) was not statistically significant ( p > 0.05 (n.s.)) in comparison to control. In these experiments, the TBARS level (marker of lipid peroxidation) in control samples (plasma treated with only H 2O 2/Fe) was 0.341 ± 0.078 nmol/mL of plasma. Nelson Hemostasis Leaf Oxidative stress Phenolic compounds Twig.Įffects of SBT twig and leaf extracts (0.5–50 μg/mL 15 min ( a) and 60 min ( b)) on plasma lipid peroxidation induced by H 2O 2/Fe. Moreover, it is very important from an economic point of view to know that there is a possibility of obtaining phenolic compounds not only from the berries or leaves, but also from twigs, which constitute a production waste.īerry Elaeagnus rhamnoides (L.) a. Our present results have demonstrated that extracts from different parts of SBT, especially berries and twigs, in comparison to well-known berries (aronia and grape), may also be viewed as a good source of active substances - antioxidants for pharmacological or cosmetic applications. Both extracts also showed anticoagulant properties. SBT twig and leaf extracts were observed to exhibit an antioxidant activity against two strong biological oxidants: hydrogen peroxide (H 2O 2) and H 2O 2/Fe (the donor of hydroxyl radicals), which induced human plasma lipid peroxidation and protein carbonylation. Additionally, we analysed the potential mechanism of modulation of hemostatic properties of human plasma (using selected coagulation times). the level of carbonyl groups in proteins. We determined the effect of plant extracts on the oxidative stress using selected markers of this process, i.e. Moreover, the aim of present experiments was to compare the biological activity of SBT leaf extract and SBT twig extract with selected berry extracts (a rich source of phenolic compounds): SBT berry extract (flavonoids being the dominant components), a commercial extract from the berries of Aronia melanocarpa (Aronox®), and a grape seed extract. The present study was carried out in order to investigate antioxidant and anticoagulant properties of sea buckthorn twig and leaf extracts (0.5-50 μg/mL) by using various in vitro models. However, not only sea buckthorn berries, but also leaves of this plant (both fresh and dried) contain a lot of nutrients and bioactive compounds, including phenolic compounds. Different bioactive compounds in SBT berries are of special interest to various researchers. Nelson, SBT) is a valuable plant because of its medical and therapeutic potential. Leaflets with short acute or blunt tips fruits smaller (2.5 to 5.Sea buckthorn (Elaeagnus rhamnoides (L.) A. Leaflets with long, tapered tips fruits very large (4 to 7.5 cm long) and narrowly oblongĥ. Lateral leaflets acute at base leaflet stalk without wingsĥ. Lateral leaflets gradually narrowed at base into a narrow wing that runs down the upper part of the leaflet stalkĤ. Young branches more-or-less circular in cross-sectionĤ. Here is a quick key to help distinguish among them during the growing season:ġ. In our primary target region, there are six, widely recognized native Fraxinus species. How to identify Eastern North American Fraxinus species
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